Journal: PLoS Neglected Tropical Diseases

Article Title: HSP70 Domain II of Mycobacterium tuberculosis Modulates Immune Response and Protective Potential of F1 and LcrV Antigens of Yersinia pestis in a Mouse Model

doi: 10.1371/journal.pntd.0003322

Figure Lengend Snippet: Cytokines expressed by splenocytes collected from mice immunized with F1, F1+HSP70(II), LcrV, LcrV+HSP70(II), F1+LcrV+HSP70(II) and HSP70(II) including control group were measured. Concentrations of cytokines detected in splenocytes supernatant after 48 h of stimulation with specific antigens (5 µg/ml) are shown. Graphs showed concentrations of (A) IL-2, (B) IFN-γ, (C) TNF-α in picograms per millilitre (pg/ml). Each bar represents the average of 8 mice/group ± S.D and is representative of three independent experiments. Analysis was done by one way ANOVA, All Pairwise Multiple Comparison Procedure (Fisher LSD Method). *P<0.05; **P<0.01; ***P<0.001; # P<0.001.

Article Snippet: After permeabilization with BD cytofix/cytoperm Kit (BD Biosciences), cells were treated with PE labelled rat anti-mouse IFN-γ monoclonal antibodies (BD Biosciences) for 30 min at room temperature in dark.

Techniques:

Journal: PLoS Neglected Tropical Diseases

Article Title: HSP70 Domain II of Mycobacterium tuberculosis Modulates Immune Response and Protective Potential of F1 and LcrV Antigens of Yersinia pestis in a Mouse Model

doi: 10.1371/journal.pntd.0003322

Figure Lengend Snippet: Graphical representation showing the significant difference in the percentage of IFN-γ producing CD4+ T cells in the spleen of different immunized animal groups after stimulation with specific antigens in comparison to the PBS control group. Each bar represents the average of 3 mice/group ± S.D [B]. Analysis was done by one way ANOVA, All Pairwise Multiple Comparison Procedure (Fisher LSD Method). **P<0.01; ***P<0.001; # P<0.001.

Article Snippet: After permeabilization with BD cytofix/cytoperm Kit (BD Biosciences), cells were treated with PE labelled rat anti-mouse IFN-γ monoclonal antibodies (BD Biosciences) for 30 min at room temperature in dark.

Techniques:

Journal: PLoS Neglected Tropical Diseases

Article Title: HSP70 Domain II of Mycobacterium tuberculosis Modulates Immune Response and Protective Potential of F1 and LcrV Antigens of Yersinia pestis in a Mouse Model

doi: 10.1371/journal.pntd.0003322

Figure Lengend Snippet: Graphical representation showing the significant difference in the percentage of IFN-γ producing CD8+ T cells in the spleen of different immunized animal groups after stimulation with specific antigens in comparison to the PBS control group. Each bar represents the average of 3 mice/group ± S.D [B]. Analysis was done by one way ANOVA, All Pairwise Multiple Comparison Procedure (Fisher LSD Method). *P<0.05; **P<0.01; ***P<0.001; # P<0.001.

Article Snippet: After permeabilization with BD cytofix/cytoperm Kit (BD Biosciences), cells were treated with PE labelled rat anti-mouse IFN-γ monoclonal antibodies (BD Biosciences) for 30 min at room temperature in dark.

Techniques: